Shreya Das

Emerging Researcher in Regenerative Medicine & Molecular Biology

Specializing in CRISPR based diagnostics, disease model systems, cellular assays and translational biology.

I work at the intersection of molecular analysis, bioinformatics and disease biology. My projects focus on CRISPR-driven antimicrobial detection, liver fibrosis modelling, and the molecular pathways driving cancer progression.

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About Me

Professional Summary

I am an early-career researcher with interdisciplinary experience in molecular biology, tissue engineering, liver fibrosis, cancer progression, and CRISPR-based pathogen detection. Across my work, I aim to bridge experimental research with computational interpretation to understand disease mechanisms at the molecular level. My research has involved investigating SOX9-mediated liver repair, exploring ferroptosis in hepatocellular carcinoma, developing CRISPR-Cas12 diagnostic assays for antimicrobial resistance, and analyzing mitochondrial sirtuins through structural bioinformatics.

With hands-on experience in various laboratory techniques and computational analysis, I bridge the gap between wet lab and dry lab research.

Liver Fibrosis SOX9 Mechanisms Computational Biology Tissue Engineering

Education

PGCert Tissue Engineering for Regenerative Medicine

University of Manchester, UK | 2025

MSc Biological Science

Presidency University, WB, India | 2023

BSc Biological Sciences

Presidency University, WB, India | 2021

Research Experience

CRISPR Diagnostics

CRISPR-Based MRSA Detection

Developing a rapid CRISPR–Cas12 diagnostic system for detecting Methicillin-Resistant Staphylococcus aureus (MRSA).

Liver Research

Liver Fibrosis & HCC Progression

Investigated SOX9 role in liver repair using HepG2 cells through various molecular biology techniques.

Bioinformatics

Mitochondrial Sirtuins Thesis

Structural bioinformatics analysis of SIRT3/4/5 proteins using computational tools and modeling.

Molecular Biology

Additional Research Projects

Various molecular biology techniques including PCR, Western Blot, and Cell Culture.

Advanced Molecular & Cellular Biology Research

Duration: 2021-2023

Institution: Presidency University, Kolkata

Research Projects:

1. Flow Cytometry Analysis

Sample: Human peripheral blood
Analysis: T-cell subset quantification
Results:

CD4+ T-cells: 52%
CD8+ T-cells: 30%
Regulatory T-cells: 7%
CD4/CD8 Ratio: 1.7 (average)

2. Histopathological Assessment

Model: Rat liver tissue toxicity
Techniques: H&E staining, Trichrome staining
Focus: Tissue morphological changes under experimental conditions

3. Cellular Motility Studies

Tissue: Isolated rat small intestine
Parameters Tested:

Ion concentration effects
Neurotransmitter impacts
Temperature variation responses

4. Journal Club Excellence

Achievement: Highest grade among 30 participants
Paper Analyzed: 'Bordetella Acetylome is shaped by Lysine Deacetylase Bkd1'
Skills Demonstrated: Critical analysis, scientific presentation

5. Grant Proposal Development

Project: 'Regulation of VLCAD activity by SIRT5 under Calorie Restriction'
Budget: 1.3 million INR successfully drafted
Scope: Metabolic regulation research proposal

Techniques Mastered:

Flow Cytometry (Experimental design → Data analysis)
Histological Staining (H&E, Trichrome)
In vitro tissue analysis
Scientific paper critical appraisal
Research grant writing

Cytochemical Localization of Biomolecules

Duration: 2018-2021 • BSc Research Project

Institution: Presidency University, Kolkata • CGPA: 8.55/10

Project Overview:

Performed comprehensive cytochemical staining of mammalian epithelial cells to localize and distinguish cellular biomolecules.

Methodology:

DNA Localization: Feulgen staining technique
RNA Identification: Methyl Green-Pyronin staining
Protein Detection: Bromophenol Blue staining
Visualization: Light microscopy analysis
Compartment Analysis: Nuclear vs cytoplasmic distribution

Additional Techniques:

Histone Staining: Fast Green method
Polysaccharide Detection: Periodic Acid-Schiff (PAS)
Mitochondrial Staining: Janus Green B vital staining
Bacterial Staining: Various morphological techniques

Nucleic Acid Quantification:

DNA Estimation: Diphenylamine (DPA) method
RNA Quantification: Orcinol reaction protocol

Molecular Biology Skills:

Chromosomal & plasmid DNA isolation from E. coli
Restriction enzyme digestion protocols
DNA fragment size estimation via gel electrophoresis
Molecular marker applications

Achievement:

Successfully demonstrated selective staining patterns and compiled comprehensive results in documented report with oral presentation.

Shreya Das

Specializing in CRISPR based diagnostics, disease model systems, cellular assays and translational biology.

Structural Features of Mitochondrial Sirtuins and Inhibition Mechanisms

About Me

Professional Summary

Education

PGCert Tissue Engineering for Regenerative Medicine

MSc Biological Science

BSc Biological Sciences

Research Experience

CRISPR-Based MRSA Detection

CRISPR-Based MRSA Detection

Liver Fibrosis & HCC Progression

Liver Fibrosis & Hepatocellular Carcinoma (HCC) Progression

Mitochondrial Sirtuins Thesis

Additional Research Projects

Technical Skills

Laboratory Techniques

Bioinformatics

Molecular Biology

Data Analysis

Certifications

PCR Certification

Nanobiotechnology

Cancer Transcriptomics Data Analysis

RNA-Based Therapeutics

CRISPR Fundamentals

Get In Touch

Contact Information

Email

Phone

Location

Connect With Me

Advanced Molecular & Cellular Biology Research

Research Projects:

1. Flow Cytometry Analysis

2. Histopathological Assessment

3. Cellular Motility Studies

4. Journal Club Excellence

5. Grant Proposal Development

Techniques Mastered:

Cytochemical Localization of Biomolecules

Project Overview:

Methodology:

Additional Techniques:

Nucleic Acid Quantification:

Molecular Biology Skills:

Achievement: